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KMID : 0545120020120030490
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 3 p.490 ~ p.496
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Medium Optimization for Phytase Production by Recombinant Escherichia coli Using Statistical Experimental Design
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Choi, Won Chan
Oh, Byung Chul/Kim, Hyung Kwoun/Lee, Eun Sook/Oh, Tae Kwang
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Abstract
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The production of E. coli WC7 phytase from a recombinant E. coli strain was optimized using a statistical experimental design approach. Two-level complete factorial designs with seven variables were used for the media optimization. In the first optimization step, the influence of disodium succinate, yeast extract, K_2HPO_4, NH_4H_2PO_4 MgSO_4, NaCl, and trace elements on phytase production was evaluated. As a result, disodium succinate, yeast extract, NH_4H_2PO_4, NaCl, and the trace elements were found to have a positive influence on the phytase production, while K_2HPO_4 and MgSO_4 had a negative influence. In the second step, the concentrations of disodium succinate and yeast extract were further optimized using central composite designs. The maximum phytase activity obtained was 234 U/ml using 15.9 g/l disodium succinate, 20 g/l yeast extract, 5 g/1 K_2HPO_4, 10 g/1 NH_4H_2PO_4, 1.5 g/l MgSO_4 4g/l NaCl, and 1.5 ml/l trace elements, which was about a 14-fold increase in comparison with that obtained using the basal medium.
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